46th Meeting of Ecological Society of Japan (1999), Shinshu University
1Laboratory of Ethology, Department of Veterinary Medicine, Tokyo University of Agriculture and Technology
2Yakult Laboratory
ABSTRACT. Total DNA was individually extracted from the butterflies, Sasakia charonda (Nymphalidae) and Pieris rapae crucivora (Pieridae). D-loop region of the mitochondrial DNA was amplified by PCR, and the sequence was determined. For S. charonda, 12 haplotypes were discriminated. The proportion of individuals which shared the same haplotypes was very high between Hirai and Ome areas (west Tama) compared to those among more distant areas, such as Miyazaki, Gifu and Shizuoka prefecture. On the other hand, 66 haplotypes were discriminated for P. rapae crucivora in west Tama. The most frequent haplotype was always most dominant in all three areas (14-29%), and other haplotypes were often local-specific and relatively rare in total area.
Keywords: Insect, Lepidoptera, Conservation biology, mtDNA